Diversity of Endophytic Fungal Community in Leaves of Artemisia argyi Based on High-throughput Amplicon Sequencing.

To investigate the community structure and diversity of endophytic fungi in the leaves of Artemisia argyi, leaf samples were collected from five A. argyi varieties grown in different cultivation areas in China, namely, Tangyin Beiai in Henan (BA), Qichun Qiai in Hubei (QA), Wanai in Nanyang in Henan (WA), Haiai in Ningbo in Zhejiang (HA), and Anguo Qiai in Anguo in Hebei (AQA), and analyzed using Illumina high-throughput sequencing technology. A total of 365,919 pairs of reads were obtained, and the number of operational taxonomic units for each sample was between 165 and 285. The alpha diversity of the QA and BA samples was higher, and a total of two phyla, eight classes, 12 orders, 15 families, and 16 genera were detected. At the genus level, significant differences were noted in the dominant genera among the samples, with three genera being shared in all the samples. The dominant genus in QA was Erythrobasidium, while that in AQA, HA, and BA was Sporobolomyces, and that in WA was Alternaria, reaching a proportion of 16.50%. These results showed that the fungal community structure and diversity in QA and BA were high. The endophytes are of great importance to the plants, especially for protection, phytohormone and other phytochemical production, and nutrition. Therefore, this study may be significant with the industrial perspective of Artemisia species.

[Effect of polyethyleneimine on the morphology of ZnO nanorod arrays].

Highly ordered ZnO nanorod arrays were successfully synthesized on glass substrates coated by the ZnO seeding via a chemical method, and the effect of the polyethylenei mine(PEI) on the structural properties and morphology of ZnO nanorod arrays was investigated. The XRD and FESEM results showed that the ZnO nonorods were wurtzite structure, which was hexagonal in shape. And the nanorods grew densely and vertically on the substrate. With the PEI amount increasing, the average diameter of the nanorods decreased, and the growth rate was slower. The Raman spectra showed that the oxygen deficiency in the ZnO lattice reduced with the increase in PEI concentration. Finally, possible mechanisms of the effect of PEI concentration on the ZnO nanorod structure were discussed.